【SUMMARY】
Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world, and causes up to 100 million infections annually. Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia and rash. Primary Dengue infection causes IgM antibodies to increase to a detectable level in 3 to 5 days after the onset of fever. IgM antibodies generally persist for 30 to 90 days. 3 Most Dengue patients in endemic regions have secondary infections, 4 resulting in high levels of specific IgG antibodies prior to or simultaneous with IgM response. 5 Therefore, the detection of specific anti-Dengue IgM and IgG antibodies can also help to distinguish between primary and secondary infections.
The Dengue Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test that utilizes a combination of Dengue antigen coated colored particles for the detection of IgG and IgM Dengue antibodies in human whole blood, serum, or plasma.

【DIRECTIONS FOR USE】
Allow the test cassette, specimen, buffer, and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface.
· For Serum or Plasma Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 5 μl), and transfer the specimen to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40ul) and start the timer. Avoid trapping air bubbles in the specimen well.
To use a micropipette: Pipette and dispense 5 µl of specimen to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.
· For Whole Blood (Venipuncture/Fingerstick) Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen about 1cm above the Fill Line,
and transfer 1 drop of whole blood (approximately 10 µl) to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40 ul) and start the timer.
To use a micropipette: Pipette and dispense 10 µl of whole blood to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.
3. Wait for the colored line(s) to appear. The test result should be read at 10 minutes. Do not interpret the result after 20 minutes.