【SUMMARY】
The N-terminal of the prohormone brain natriuretic peptide (NT-proBNP) is a 76 amino acid N-terminal inactive protein that is cleaved from proBNP to release brain natriuretic peptide.Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome. The plasma concentrations of both BNP and NT-proBNP are also typically increased in patients with asymptomatic or symptomatic left ventricular dysfunction and is associated with coronary artery disease and myocardial ischemia. 
The NT-proBNP Rapid Test Cassette (Whole Blood/Serum/Plasma) is a simple test that utilizes a combination of anti-NT-proBNP antibody coated particles and capture reagents to qualitatively detect NT-proBNP in whole blood, serum or plasma. The minimum detection level is 0.45ng/mL.

【DIRECTIONS FOR USE】
Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the cassette on a clean and level surface.
For Serum or Plasma specimen:
· Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75µL) to the sample well of the test cassette, then start the timer immediately. See illustration below.
For Venipuncture Whole Blood specimen:
· Hold the dropper vertically and transfer 3 drops of whole blood (approximately 75µL) to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40µL), and start the timer. See illustration below.
For Fingerstick Whole Blood specimen:
· To use a capillary tube: Fill the capillary tube and transfer approximately 75µL of fingerstick whole blood specimen to the specimen well of test cassette, then add 1 drop of buffer (approximately 40µL) and start the timer. See illustration below.
· To use hanging drops: Allow 3 hanging drops of fingerstick whole blood specimen (approximately 75 µL) to fall into the specimen well of test cassette, then add 1 drop of buffer (approximately 40µL) and start the timer. See illustration below.
3. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes.